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B Cell Memory is directed toward conformational epitopes of parvovirus B19 capsid proteins and the unique region of VP1

Mahon, Bernard and Doyle, Sean and Corcoran, Amanda (2004) B Cell Memory is directed toward conformational epitopes of parvovirus B19 capsid proteins and the unique region of VP1. The Journal of Infectious Diseases, 189. pp. 1873-80.

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Abstract

Background. Loss of antibody reactivity against linear epitopes of parvovirus B19 (B19) capsid proteins VP1 and VP2 occurs after infection; however, it is unclear whether B cell memory is established against linear epitopes. Methods. B cell enzyme-linked immunospot assay was used to evaluate B19-specific B cell memory in volunteer donors ( ). np22 Results. B cell memory is maintained against conformational epitopes of VP2 and is absent against linear epitopes of VP2. Individuals seronegative for IgG against the unique region of VP1 have detectable B cell memory, with the potential to mount a humoral response on reexposure to B19. Conversely, in mice immunized with VP2, long-lasting IgG against linear epitopes of VP2 and a strong B cell memory response are observed. Conclusions. B cell memory is established and maintained against conformational epitopes of VP2 and against linear epitopes of VP1 but not against linear epitopes of VP2. These findings further our understanding of the immune response to B19 and suggest that analysis of B19-specific B cell memory merits consideration for future B19-vaccine studies.

Item Type: Article
Keywords: B Cell memory; conformational epitodes; parvovirus B19 capside proteins; vp1
Subjects: Science & Engineering > Biology
Science & Engineering > Institute of Immunology
Item ID: 159
Depositing User: Dr. Bernard Mahon
Date Deposited: 16 Dec 2004
Journal or Publication Title: The Journal of Infectious Diseases
Publisher: Infectious Diseases Society of America
Refereed: Yes
URI:

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