Doyle, Dr. Sean and Daly, Dr. P. and Collier, Dr. T.
PCR-ELISA detection of Escherichia coli in milk.
Letters in Applied Microbiology, 34.
Aims: The purpose of this study was to develop a reliable molecular procedure for the
detection of Escherichia coli in milk.
Methods and Results: Robust and expeditious DNA extraction and PCR techniques were
evaluated using Enzyme-Linked Immunosorbent Assay (ELISA) detection of biotin-labelled
amplicons to facilitate optimal detection of E. coli DNA.
Conclusions: It was found that 5 E. coli colony-forming units (cfu) could be detected per PCR
reaction using the PCR-ELISA system, equating to a sensitivity of detection of 100 E. coli
cfu ml)1 pasteurized milk.
Significance and Impact of the Study: This approach should facilitate evaluation of milk
contamination and enable rapid detection of E. coli mastitis, leading to correct deployment of
relevant antibiotic therapy and improved animal welfare.
||PCR-ELISA; Escherichia coli; milk;
||Science & Engineering > Biology
Dr. Sean Doyle
||22 Dec 2004
|Journal or Publication Title:
||Letters in Applied Microbiology
||The Society for Applied Microbiology
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