PCR-ELISA detection of Escherichia coli in milk

Doyle, Sean and Daly, Paul and Collier, T. (2002) PCR-ELISA detection of Escherichia coli in milk. Letters in Applied Microbiology, 34 (3). pp. 222-226. ISSN 0266-8254

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Aims: The purpose of this study was to develop a reliable molecular procedure for the detection of Escherichia coli in milk. Methods and Results: Robust and expeditious DNA extraction and PCR techniques were evaluated using Enzyme-Linked Immunosorbent Assay (ELISA) detection of biotin-labelled amplicons to facilitate optimal detection of E. coli DNA. Conclusions: It was found that 5 E. coli colony-forming units (cfu) could be detected per PCR reaction using the PCR-ELISA system, equating to a sensitivity of detection of 100 E. coli cfu ml)1 pasteurized milk. Significance and Impact of the Study: This approach should facilitate evaluation of milk contamination and enable rapid detection of E. coli mastitis, leading to correct deployment of relevant antibiotic therapy and improved animal welfare.

Item Type: Article
Keywords: PCR-ELISA; Escherichia coli; milk;
Academic Unit: Faculty of Science and Engineering > Biology
Item ID: 164
Identification Number: 10.1046/j.1472-765x.2002.01074.x
Depositing User: Dr. Sean Doyle
Date Deposited: 22 Dec 2004
Journal or Publication Title: Letters in Applied Microbiology
Publisher: The Society for Applied Microbiology
Refereed: Yes
Funders: Enterprise Ireland (EI), Tridelta Development Limited

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