Doyle, Sean and Daly, Paul and Collier, T.
PCR-ELISA detection of Escherichia coli in milk.
Letters in Applied Microbiology, 34 (3).
Aims: The purpose of this study was to develop a reliable molecular procedure for the
detection of Escherichia coli in milk.
Methods and Results: Robust and expeditious DNA extraction and PCR techniques were
evaluated using Enzyme-Linked Immunosorbent Assay (ELISA) detection of biotin-labelled
amplicons to facilitate optimal detection of E. coli DNA.
Conclusions: It was found that 5 E. coli colony-forming units (cfu) could be detected per PCR
reaction using the PCR-ELISA system, equating to a sensitivity of detection of 100 E. coli
cfu ml)1 pasteurized milk.
Significance and Impact of the Study: This approach should facilitate evaluation of milk
contamination and enable rapid detection of E. coli mastitis, leading to correct deployment of
relevant antibiotic therapy and improved animal welfare.
Repository Staff Only(login required)
||Item control page