Optimised procedures for the cryopreservation of different species of Heterorhabditis

Nugent, Miriam J. and O'Leary, Sean A. and Burnell, Ann M. (1996) Optimised procedures for the cryopreservation of different species of Heterorhabditis. Fundalmental Applied Nematology, 19 (1). pp. 1-6.

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It is demonstrated that the protocols developed for the cryopreservation of Heterorhabditis bacteriophora could be successfully applied to strains of the H. bacteriophora species complex and to members of the North West European species of Heterorhabditis. However the currently available protocols were not suitable for cryopreserving the Irish or the tropical Heterorhabditis species. Therefore, the postcryopreservation viabilities of Heterorhabditis species in response to preincubation in a range of cryoprotectants at varying concentrations and incubation times were determined. Species and strain specific differences in cryopreservation response were observed among Heterorhabditis isolates and optimal conditions were developed for the cryopreservation of Irish, NW European and tropical isolates of Heterorhabditis. These optimum conditions are described in this paper.

Item Type: Article
Keywords: Heterorhabditis; cryopreservation; glycerol; dimethyl sulfoxide; desiccation; nematodes;
Academic Unit: Faculty of Science and Engineering > Biology
Item ID: 186
Depositing User: Prof. Ann Burnell
Date Deposited: 26 Jan 2005
Journal or Publication Title: Fundalmental Applied Nematology
Publisher: Gauthier-Villars
Refereed: Yes

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