A Phylogenetic Analysis of Heterorhabditis (Nemata :Rhabditidae) Based on Internal Transcribed Spacer 1 DNA Sequence Data

Adams, B.J. and Burnell, A.M. and Powers, T.O. (1998) A Phylogenetic Analysis of Heterorhabditis (Nemata :Rhabditidae) Based on Internal Transcribed Spacer 1 DNA Sequence Data. Journal of Nematology, 30 (1). pp. 22-39.

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Internal transcribed spacer 1 sequences were used to infer phylogenetic relationships among 8 of the 9 described species and one putative species of the entomoparhogenic nematode genus Heterorhabditb. Sequences were aligned and optimized based on painvise genetic distance and parsimony criteria and subjected to a variety of sequence alignment parameters. Phylogenetic trees were constructed with maximum parsimony, cladistic, distance, and maximum likelihood algorithms. Our results gave strong support for four pairs of sister species, while relationships between these pairs also were resolved but less well supported. The ITS1 region of the nuclear ribosomal repeat was a reliable source of homologous characters for resolving relationships between closely related taxa but provided more tenuous resolution among more divergent lineages. A high degree of sequence identity and lack of autapomorphic characters suggest that sister species pairs within three distinct lineages may be mutually conspecific. Application of these molecular data and current morphological knowledge to the delimitation of species is hindered by an incomplete understanding of their variability in natural populations.

Item Type: Article
Keywords: Entomopathogenic Nematode; Evolution; Heterorhabditis; ITS1; Nematode; Phylogenetic analysis; Ribosomal DNA; Species concepts;
Academic Unit: Faculty of Science and Engineering > Biology
Item ID: 187
Depositing User: Prof. Ann Burnell
Date Deposited: 26 Jan 2005
Journal or Publication Title: Journal of Nematology
Publisher: The Society of Nematologists
Refereed: Yes

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