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Electrophoretic field gradient focusing with on-column detection by fluorescence quenching

Ansell, Richard J. and Tunon, Pilar Gonzalez and Wang, Yating and Myers, Peter and Ivory, Cornelius F. and Keen, Jeffrey N. and Findlay, John B. C. (2009) Electrophoretic field gradient focusing with on-column detection by fluorescence quenching. Analyst, 134. pp. 226-229. ISSN 0003-2654

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Abstract

Native, uncoloured, proteins can be focused in a column containing a fluorescent packing material, using hydrodynamic flow and a counteracting non-linear electric field, and imaged along the length of the channel by fluorescence quenching.

Item Type: Article
Keywords: Electrophoretic; field gradient focusing; on-column detection; fluorescence quenching;
Subjects: Science & Engineering > Biology
Item ID: 2070
Depositing User: Professor John Findlay
Date Deposited: 10 Aug 2010 15:06
Journal or Publication Title: Analyst
Publisher: Royal Society of Chemistry
Refereed: Yes
URI:

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