Calia, Giammario and Rocchitta, Gaia and Migheli, Rossana and Puggioni, Giulia and Spissu, Ylenia and Bazzu, Gianfranco and Mazzarello, Vittorio and Lowry, John P. and O'Neill, Robert D. and Desole, Maria S. and Serra, Pier A.
Biotelemetric Monitoring of Brain Neurochemistry in Conscious
Rats Using Microsensors and Biosensors.
Sensors, 9 (4).
In this study we present the real-time monitoring of three key brain
neurochemical species in conscious rats using implantable amperometric electrodes
interfaced to a biotelemetric device. The new system, derived from a previous design, was
coupled with carbon-based microsensors and a platinum-based biosensor for the detection
of ascorbic acid (AA), O2 and glucose in the striatum of untethered, freely-moving rats.
The miniaturized device consisted of a single-supply sensor driver, a current-to-voltage
converter, a microcontroller and a miniaturized data transmitter. The redox currents were
digitized to digital values by means of an analog-to-digital converter integrated in a
peripheral interface controller (PIC), and sent to a personal computer by means of a
miniaturized AM transmitter. The electronics were calibrated and tested in vitro under
different experimental conditions and exhibited high stability, low power consumption and
good linear response in the nanoampere current range. The in-vivo results confirmed previously published observations on striatal AA, oxygen and glucose dynamics recorded
in tethered rats. This approach, based on simple and inexpensive components, could be
used as a rapid and reliable model for studying the effects of different drugs on brain
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