Skeletal muscle proteomics: current approaches,
technical challenges and emerging techniques.
Skeletal Muscle, 1 (6).
Background: Skeletal muscle fibres represent one of the most abundant cell types in mammals. Their highly
specialised contractile and metabolic functions depend on a large number of membrane-associated proteins with
very high molecular masses, proteins with extensive posttranslational modifications and components that exist in
highly complex supramolecular structures. This makes it extremely difficult to perform conventional biochemical
studies of potential changes in protein clusters during physiological adaptations or pathological processes.
Results: Skeletal muscle proteomics attempts to establish the global identification and biochemical characterisation
of all members of the muscle-associated protein complement. A considerable number of proteomic studies have
employed large-scale separation techniques, such as high-resolution two-dimensional gel electrophoresis or liquid
chromatography, and combined them with mass spectrometry as the method of choice for high-throughput
protein identification. Muscle proteomics has been applied to the comprehensive biochemical profiling of
developing, maturing and aging muscle, as well as the analysis of contractile tissues undergoing physiological
adaptations seen in disuse atrophy, physical exercise and chronic muscle transformation. Biomedical investigations
into proteome-wide alterations in skeletal muscle tissues were also used to establish novel biomarker signatures of
neuromuscular disorders. Importantly, mass spectrometric studies have confirmed the enormous complexity of
posttranslational modifications in skeletal muscle proteins.
Conclusions: This review critically examines the scientific impact of modern muscle proteomics and discusses its
successful application for a better understanding of muscle biology, but also outlines its technical limitations and
emerging techniques to establish new biomarker candidates.
||Research in the author’s laboratory was supported by grants from the Irish
Health Research Board, the Irish Higher Education Authority, Muscular
Dystrophy Ireland, Science Foundation Ireland and the European
Commission. The author thanks all members of the NUIM Muscle Biology
Laboratory and our many international collaborators for their help and
encouragement over the past few years.
||Skeletal muscle; muscle proteomics;
||Science & Engineering > Biology
Prof. Kay Ohlendieck
||02 Mar 2011 17:32
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