Molony, Karen M. and Ryle, James P. and McDonnell, Susan and Sheridan, John T. and Naughton, Thomas J.
Segmentation and visualization of digital in-line holographic
microscopy of three-dimensional scenes using reconstructed
Proceedings of SPIE, 7443 (74431F).
This paper demonstrates a technique that could prove useful for extracting three-dimensional (3D) models
from a single two-dimensional (2D) digital in-line holographic microscopy (DIHM) recording. Multiple intensity
images are reconstructed at a range of depths through a transmissive or partially transmissive scene recorded
by DIHM. A two step segmentation of each of these reconstructed intensity images facilitates the construction
of a data set of surfaces in 3D. First an adaptive thresholding step and then a border following step are
implemented. The surfaces of segmented features are rendered in 3D by applying the marching cubes algorithm
to polygonize the data set. Experimental results for a real world DIHM capture of a transmissive glass sample
are presented to demonstrate this segmentation and visualization process.
||Copyright 2009 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. We thank the Irish Research Council for Science, Engineering and Technology, Enterprise Ireland and Science
Foundation Ireland, under the National Development Plan for funding this research. The authors wish to thank
SPIE for supporting conference attendance through the Student Chapter Travelling Officer Grant programme.
||Segmentation; visualization; digital in-line holographic
microscopy; three-dimensional scenes; reconstructed
||Faculty of Science and Engineering > Computer Science
||13 Apr 2011 15:47
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||Proceedings of SPIE
||SPIE (Society of Photo-optical Instrumentation Engineers)
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