Ryle, James P. and Molony, Karen M. and McDonnell, Susan and Naughton, Thomas J. and Sheridan, John T.
Multispectral lensless digital holographic microscope: imaging MCF-7
and MDA-MB-231 cancer cell cultures.
Proceedings of SPIE, 7442 (744206).
Digital holography is the process where an object’s phase and amplitude information is retrieved from intensity images
obtained using a digital camera (e.g. CCD or CMOS sensor). In-line digital holographic techniques offer full use of the
recording device’s sampling bandwidth, unlike off-axis holography where object information is not modulated onto
carrier fringes. Reconstructed images are obscured by the linear superposition of the unwanted, out of focus, twin
images. In addition to this, speckle noise degrades overall quality of the reconstructed images. The speckle effect is a
phenomenon of laser sources used in digital holographic systems. Minimizing the effects due to speckle noise, removal
of the twin image and using the full sampling bandwidth of the capture device aids overall reconstructed image quality.
Such improvements applied to digital holography can benefit applications such as holographic microscopy where the
reconstructed images are obscured with twin image information. Overcoming such problems allows greater flexibility in
current image processing techniques, which can be applied to segmenting biological cells (e.g. MCF-7 and MDA-MB-
231) to determine their overall cell density and viability. This could potentially be used to distinguish between apoptotic
and necrotic cells in large scale mammalian cell processes, currently the system of choice, within the biopharmaceutical
||Copyright 2009 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. The authors acknowledge the support of Enterprise Ireland and Science Foundation Ireland. They also thank the Irish
Research Council for Science, Engineering and Technology for their support under the national development plan.
James Ryle wishes to thank SPIE for supporting conference attendance through the Student Chapter Officer Travel Grant
programme. He also acknowledges financial support from UCD’s Seed Funding Scheme.
||In-line digital holography; lensless microscope; Gabor microscopy; wavefront reconstruction;
||Faculty of Science and Engineering > Computer Science
||13 Apr 2011 15:46
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||Proceedings of SPIE
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