Molony, Karen M. and Naughton, Thomas J.
Segmentation and three-dimensional visualisation of digital in-line holographic
CIICT 2009 : proceedings of the China-Ireland information and communications technologies conference.
Dept. of Computer Science, National University of Ireland, Maynooth, Co. Kildare, Ireland, pp. 227-233.
This paper demonstrates that transmissive or partially
transmissive scenes imaged by digital in-line holographic
microscopy (DIHM) can be reconstructed as a threedimensional
(3-D) model of the imaged volume from a single
capture. This process entails numerical reconstruction,
segmentation and polygonisation. Numerical reconstruction
of a digital hologram captured using a DIHM set up
is performed at equally spaced depths within a range. In
the case of intensity modulating objects, segmentation of
each of the reconstructed intensity images produces a contour
slice of the scene by applying an adaptive threshold
and border following. These slices are visualised in 3-D by
polygonising the data using the marching cubes algorithm.
We present experimental results for a real world DIHM capture
of a partially transmissive scene that demonstrates the
steps in this process.
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