Singh, Ishwar and Vyle, Joseph S. and Heaney, Frances
Supporting Information: Fast, Copper-Free Click Chemistry, A Convenient Solid-Phase
Approach to Oligonucleotide Conjugation.
Royal Society of Chemistry.
Analytical TLC was performed on precoated (250 μm) silica gel 60 F-254 plates from Merck.
All plates were visualized by UV irradiation, and/or staining with 5% H2SO4 in ethanol
followed by heating. Flash chromatography grade silica gel 60 (230-400 mesh) was obtained
from Merck. Mass analysis was performed on an Ettan MALDI-TOF Pro from Amersham
Biosciences or LASER-TOF LT3 from Scientific Analytical Instruments with 3-
hydroxypicolinic acid or 2,’ 4’, 6’-trihydroxyacetophenone as matrix. The NMR spectra were
obtained at 1H (300 MHz), 13C (75 MHz) and 31P (121 MHz) on a Bruker instrument at 25 ºC.
Chemical shifts are reported in ppm downfield from TMS as standard. HPLC was carried out
using a Gilson instrument equipped with a UV detector and a Nucleosil C18 column (4.0 ×
250 mm) or Phenomenex Clarity. Fluorescence spectra were recorded on a Varian Cary
Eclipse instrument. All other chemical agents were purchased from Aldrich Chemical
Company unless otherwise noted.
Repository Staff Only(login required)
||Item control page