Fungal proteomics: from identitcation to function.
Microbiology Letters, 321.
Some fungi cause disease in humans and plants, while others have demonstrable
potential for the control of insect pests. In addition, fungi are also a rich reservoir
of therapeutic metabolites and industrially useful enzymes. Detailed analysis of
fungal biochemistry is now enabled by multiple technologies including protein
mass spectrometry, genome and transcriptome sequencing and advances in
bioinformatics. Yet, the assignment of function to fungal proteins, encoded either
by in silico annotated, or unannotated genes, remains problematic. The purpose of
this review is to describe the strategies used by many researchers to reveal protein
function in fungi, and more importantly, to consolidate the nomenclature of
‘unknown function protein’ as opposed to ‘hypothetical protein’ – once any
protein has been identified by protein mass spectrometry. A combination of
approaches including comparative proteomics, pathogen-induced protein expression
and immunoproteomics are outlined, which, when used in combination with
a variety of other techniques (e.g. functional genomics, microarray analysis,
immunochemical and infection model systems), appear to yield comprehensive
and definitive information on protein function in fungi. The relative advantages of
proteomic, as opposed to transcriptomic-only, analyses are also described. In the
future, combined high-throughput, quantitative proteomics, allied to transcriptomic
sequencing, are set to reveal much about protein function in fungi.
||fungal proteomics; protein mass spectrometry;
MALDI-ToF; LC-MS; Aspergillus fumigatus;
||Faculty of Science and Engineering > Biology
Dr. Sean Doyle
||27 Jan 2012 11:50
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||Blackwell Publishing Ltd.
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