Substrate-Promoted Formation of a Catalytically Competent Binuclear Center and Regulation of Reactivity in a Glycerophosphodiesterase from Enterobacter aerogenes

Hadler, Kieran S. and Tanifum, Eric A. and Hsu-Chen Yip, Sylvia and Mitic, Natasa and Guddat, Luke W. and Jackson, Colin J. and Gahan, Lawrence R. and Nguyen, Kelly and Carr, Paul D. and Ollis, David L. and Hengge, Alvan C. and Larrabee, James A. and Schenk, Gerhard (2008) Substrate-Promoted Formation of a Catalytically Competent Binuclear Center and Regulation of Reactivity in a Glycerophosphodiesterase from Enterobacter aerogenes. Journal of the American Chemical Society, 130 (43). pp. 14129-14138. ISSN 0002-7863

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The glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes is a promiscuous binuclear metallohydrolase that catalyzes the hydrolysis of mono-, di-, and triester substrates, including some organophosphate pesticides and products of the degradation of nerve agents. GpdQ has attracted recent attention as a promising enzymatic bioremediator. Here, we have investigated the catalytic mechanism of this versatile enzyme using a range of techniques. An improved crystal structure (1.9 Å resolution) illustrates the presence of (i) an extended hydrogen bond network in the active site, and (ii) two possible nucleophiles, i.e., water/hydroxide ligands, coordinated to one or both metal ions. While it is at present not possible to unambiguously distinguish between these two possibilities, a reaction mechanism is proposed whereby the terminally bound H2O/OH- acts as the nucleophile, activated via hydrogen bonding by the bridging water molecule. Furthermore, the presence of substrate promotes the formation of a catalytically competent binuclear center by significantly enhancing the binding affinity of one of the metal ions in the active site. Asn80 appears to display coordination flexibility that may modulate enzyme activity. Kinetic data suggest that the rate-limiting step occurs after hydrolysis, i.e., the release of the phosphate moiety and the concomitant dissociation of one of the metal ions and/or associated conformational changes. Thus, it is proposed that GpdQ employs an intricate regulatory mechanism for catalysis, where coordination flexibility in one of the two metal binding sites is essential for optimal activity.

Item Type: Article
Additional Information: The definitive version of this article is published at the Journal of the American Chemical Society, 2008, 130 (43), pp 14129–14138, DOI: 10.1021/ja803346w . Copyright © 2008 American Chemical Society
Keywords: Substrate-Promoted; Formation; Catalytically Competent; Binuclear Center; Regulation; Reactivity; Glycerophosphodiesterase; Enterobacter aerogenes;
Academic Unit: Faculty of Science and Engineering > Chemistry
Item ID: 3701
Depositing User: Gary Schenk
Date Deposited: 29 May 2012 15:29
Journal or Publication Title: Journal of the American Chemical Society
Publisher: American Chemical Society
Refereed: Yes

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