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Identification of a non-purple tartrate-resistant acid phosphatase: an evolutionary link to Ser/Thr protein phosphatases?

Hadler, Kieran S. and Huber, Thomas and Cassady, A. Ian and Weber, Jane and Robinson, Jodie and Burrows, Allan and Kelly, Gregory and Guddat, Luke W. and Hume, David A. and Schenk, Gerhard and Flanagan, Jack U. (2008) Identification of a non-purple tartrate-resistant acid phosphatase: an evolutionary link to Ser/Thr protein phosphatases? BMC Research Notes, 1 (78). pp. 1-8. ISSN 1756-0500

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Abstract

Background: Tartrate-resistant acid phosphatases (TRAcPs), also known as purple acid phosphatases (PAPs), are a family of binuclear metallohydrolases that have been identified in plants, animals and fungi. The human enzyme is a major histochemical marker for the diagnosis of bonerelated diseases. TRAcPs can occur as a small form possessing only the ~35 kDa catalytic domain, or a larger ~55 kDa form possessing both a catalytic domain and an additional N-terminal domain of unknown function. Due to its role in bone resorption the 35 kDa TRAcP has become a promising target for the development of anti-osteoporotic chemotherapeutics. Findings: A new human gene product encoding a metallohydrolase distantly related to the ~55 kDa plant TRAcP was identified and characterised. The gene product is found in a number of animal species, and is present in all tissues sampled by the RIKEN mouse transcriptome project. Construction of a homology model illustrated that six of the seven metal-coordinating ligands in the active site are identical to that observed in the TRAcP family. However, the tyrosine ligand associated with the charge transfer transition and purple color of TRAcPs is replaced by a histidine. Conlusion: The gene product identified here may represent an evolutionary link between TRAcPs and Ser/Thr protein phosphatases. Its biological function is currently unknown but is unlikely to be associated with bone metabolism.

Item Type: Article
Additional Information: This article is available from: http://www.biomedcentral.com/1756-0500/1/ © 2008 Schenk et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: non-purple tartrate-resistant acid phosphatase; gene; protein phosphatases; binuclear metallohydrolases;
Subjects: Science & Engineering > Chemistry
Item ID: 3709
Identification Number: 10.1186/1756-0500-1-78
Depositing User: Gary Schenk
Date Deposited: 30 May 2012 15:50
Journal or Publication Title: BMC Research Notes
Publisher: BioMed Central
Refereed: Yes
URI:

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