Maynooth University

Maynooth University ePrints and eTheses Archive

Maynooth University Library

Rapid-Freeze-Quench Magnetic Circular Dichroism of Intermediate X in Ribonucleotide Reductase: New Structural Insight

Mitic, Natasa and Saleh, Lana and Schenk, Gerhard and Bollinger, J. Martin Jr. and Solomon, Edward I. (2003) Rapid-Freeze-Quench Magnetic Circular Dichroism of Intermediate X in Ribonucleotide Reductase: New Structural Insight. Journal of the American Chemical Society, 125 (37). pp. 11200-11201. ISSN 0002-7863

[img] Download (81kB)

Abstract

To elucidate the electronic structure of intermediate X in the oxygen activation reaction of the R2 subunit of ribonucleotide reductase, a protocol has been developed to perform magnetic circular dichroism (MCD) on a rapid-freeze-quench, strain free optical sample. RFQ-MCD data have been collected on intermediate X in the double mutant of R2, Y122/Y356F. While X has been reported to exhibit a broad absorption band at 365 nm, there are at least 10 electronic transitions observed at low-temperature MCD. From C0/D0 ratios, the transitions of X can be divided into three regions: 16 000-22 000 cm-1 region involving spin-allowed ligand field transitions of the Fe(IV), 23 000-24 000 cm-1 region of spin-forbidden, spin-flip transitions on the Fe(IV), and the charge transfer (CT) region from 26 000 to 32 000 cm-1. The C0/D0 ratios from d --> d and CT transitions strongly support significant Fe(IV) character coupled into the paramagnetic center. Ligand field (spin-allowed d --> d region) analysis allows the bis-mu-oxo and mu-oxo plus other monoanionic bridge possibilities for the structure of intermediate X to be distinguished, providing new insight into the molecular mechanism of the cluster formation in R2.

Item Type: Article
Additional Information: The definitive version of this article is available at the Journal of the American Chemical Society, 2003, v.125, pp.11200-11201 . © 2003 American Chemical Society
Keywords: Rapid-Freeze-Quench; Magnetic Circular Dichroism; Intermediate X; Ribonucleotide Reductase;
Subjects: Science & Engineering > Chemistry
Item ID: 3738
Depositing User: Gary Schenk
Date Deposited: 06 Jun 2012 14:24
Journal or Publication Title: Journal of the American Chemical Society
Publisher: American Chemical Society
Refereed: Yes
URI:

Repository Staff Only(login required)

View Item Item control page

Document Downloads

More statistics for this item...