Conjugation at the oligonucleotide level based on isoxazole phosphoramidites generated by click chemistry


Freeman, Colin and Ni Cheallaigh, Aisling and Heaney, Frances (2011) Conjugation at the oligonucleotide level based on isoxazole phosphoramidites generated by click chemistry. Tetrahedron, 67 (40). pp. 7860-7865. ISSN 0040-4020

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Abstract

The versatility of the isoxazole generating nitrile oxide–alkyne Huisgen cycloaddition for provision of chemically modified oligonucleotides has been extended; in a novel approach isoxazole conjugated oligodeoxyribonucleotides have been constructed by phosphoramidite chemistry of isoxazole derivatives previously generated by nitrile oxide–alkyne click chemistry. The conjugation involves manual solid phase synthesis at room temperature in aqueous ethanol and proceeds in high yield.

Item Type: Article
Keywords: Conjugation; oligonucleotide; isoxazole phosphoramidites; click chemistry;
Academic Unit: Faculty of Science and Engineering > Chemistry
Item ID: 4292
Depositing User: Dr. Frances Heaney
Date Deposited: 09 Apr 2013 10:07
Journal or Publication Title: Tetrahedron
Publisher: Elsevier
Refereed: Yes
URI:

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