Gerke, Jennifer and Bayram, Ozgur and Feussner, Kirstin and Landesfeind, Manuel and Shelest, Ekaterina and Feussner, Ivo and Braus, Gerhard H.
(2012)
Breaking the Silence: Protein Stabilization Uncovers Silenced
Biosynthetic Gene Clusters in the Fungus Aspergillus nidulans.
Applied and Environmental Microbiology, 78 (23).
pp. 8234-8244.
ISSN 0099-2240
Abstract
The genomes of filamentous fungi comprise numerous putative gene clusters coding for the biosynthesis of chemically and
structurally diverse secondary metabolites (SMs), which are rarely expressed under laboratory conditions. Previous approaches
to activate these genes were based primarily on artificially targeting the cellular protein synthesis apparatus. Here, we applied an
alternative approach of genetically impairing the protein degradation apparatus of the model fungus Aspergillus nidulans by
deleting the conserved eukaryotic csnE/CSN5 deneddylase subunit of the COP9 signalosome. This defect in protein degradation
results in the activation of a previously silenced gene cluster comprising a polyketide synthase gene producing the antibiotic 2,4-
dihydroxy-3-methyl-6-(2-oxopropyl)benzaldehyde (DHMBA). The csnE/CSN5 gene is highly conserved in fungi, and therefore,
the deletion is a feasible approach for the identification of new SMs.
| Item Type: |
Article
|
| Additional Information: |
Supplemental material for this article may be found at http://aem.asm.org/. |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
6246 |
| Identification Number: |
https://doi.org/10.1128/AEM.01808-12 8234 |
| Depositing User: |
Ozgur Bayram
|
| Date Deposited: |
13 Jul 2015 15:20 |
| Journal or Publication Title: |
Applied and Environmental Microbiology |
| Publisher: |
American Society for Microbiology |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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