Breaking the Silence: Protein Stabilization Uncovers Silenced Biosynthetic Gene Clusters in the Fungus Aspergillus nidulans


Gerke, Jennifer and Bayram, Ozgur and Feussner, Kirstin and Landesfeind, Manuel and Shelest, Ekaterina and Feussner, Ivo and Braus, Gerhard H. (2012) Breaking the Silence: Protein Stabilization Uncovers Silenced Biosynthetic Gene Clusters in the Fungus Aspergillus nidulans. Applied and Environmental Microbiology, 78 (23). pp. 8234-8244. ISSN 0099-2240

[img]
Preview
Download (2MB) | Preview


Share your research

Twitter Facebook LinkedIn GooglePlus Email more...



Add this article to your Mendeley library


Abstract

The genomes of filamentous fungi comprise numerous putative gene clusters coding for the biosynthesis of chemically and structurally diverse secondary metabolites (SMs), which are rarely expressed under laboratory conditions. Previous approaches to activate these genes were based primarily on artificially targeting the cellular protein synthesis apparatus. Here, we applied an alternative approach of genetically impairing the protein degradation apparatus of the model fungus Aspergillus nidulans by deleting the conserved eukaryotic csnE/CSN5 deneddylase subunit of the COP9 signalosome. This defect in protein degradation results in the activation of a previously silenced gene cluster comprising a polyketide synthase gene producing the antibiotic 2,4- dihydroxy-3-methyl-6-(2-oxopropyl)benzaldehyde (DHMBA). The csnE/CSN5 gene is highly conserved in fungi, and therefore, the deletion is a feasible approach for the identification of new SMs.

Item Type: Article
Additional Information: Supplemental material for this article may be found at http://aem.asm.org/.
Academic Unit: Faculty of Science and Engineering > Biology
Item ID: 6246
Identification Number: 10.1128/AEM.01808-12 8234
Depositing User: Ozgur Bayram
Date Deposited: 13 Jul 2015 15:20
Journal or Publication Title: Applied and Environmental Microbiology
Publisher: American Society for Microbiology
Refereed: Yes
URI:

Repository Staff Only(login required)

View Item Item control page

Document Downloads

More statistics for this item...