An eight-subunit COP9 signalosome with an intact JAMM motif is required for fungal fruit body formation


Busch, Silke and Schwier, Elke U. and Nahlik, Krystyna and Bayram, Ozgur and Helmstaedt, Kerstin and Draht, Oliver W. and Krappmann, Sven and Valerius, Oliver and Lipscomb, William N. and Braus, Gerhard H. (2007) An eight-subunit COP9 signalosome with an intact JAMM motif is required for fungal fruit body formation. Eukaryotic Cell, 104 (19). pp. 8089-8094. ISSN 1535-9778

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Abstract

Fruit body formation in filamentous fungi is a complex and yet hardly understood process. We show here that protein turnover control is crucial for Aspergillus nidulans development. Deletion of genes encoding COP9 signalosome (CSN) subunits 1, 2, 4, or 5 resulted in identical blocks in fruit body formation. The CSN multiprotein complex controls ubiquitin-dependent protein degradation in eukaryotes. Six CSN subunits interacted in a yeast two-hybrid analysis, and the complete eight-subunit CSN was recruited by a functional tandem affinity purification tag fusion of subunit 5 (CsnE). The tagged CsnE was unable to recruit any CSN subunit in a strain deleted for subunit 1 or subunit 4. Mutations in the JAMM metalloprotease core of CsnE resulted in mutant phenotypes identical to those of csn deletion strains. We propose that a correctly assembled CSN including a functional JAMM links protein turnover to fungal sexual development.

Item Type: Article
Additional Information: This article contains supporting information online at www.pnas.org/cgi/content/full/ 0702108104/DC1.
Keywords: development, filament fungi; eight-subunit COP9; signalosome; intact JAMM motif; fungal fruit body; formation;
Academic Unit: Faculty of Science and Engineering > Biology
Item ID: 6248
Depositing User: Ozgur Bayram
Date Deposited: 14 Jul 2015 15:28
Journal or Publication Title: Eukaryotic Cell
Publisher: American Society for Microbiology
Refereed: Yes
URI:

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