Fitzpatrick, David A. and McInerney, James O.
Evidence of Positive Darwinian Selection in Omp85, a Highly Conserved
Bacterial Outer Membrane Protein Essential for Cell Viability.
Journal of Molecular Evolution, 61 (1).
Omp85is a highly conserved outer
membrane protein found in all gram-negative bacteria.
It is essential for bacterial cell viability and plays
an integral function in the positioning and folding of
other outer membrane proteins into the bacterial
outer membrane. We have employed a maximum
likelihood and a maximum parsimony approach to
detect evidence of positive Darwinian selection in
Omp85hom ologues from 10 d-proteobacteria and
have identified 14 amino acid sites that show evidence
of being under the influence of adaptive evolution.
Interestingly all sites bar one are concentrated within
surface loops of the protein that most likely interact
with host immune response or the surrounding
environment. Alternatively amino acids within
membrane-spanning regions of the protein are found
to be under purifying selection most likely as a result
of structural constraints.
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