Browne, Niall and Hackenberg, Frauke and Streciwilk, Wojciech and Tacke, Matthias and Kavanagh, Kevin
(2014)
Assessment of in vivo antimicrobial activity of the carbene
silver(I) acetate derivative SBC3 using Galleria mellonella
larvae.
Biometals, 27 (4).
pp. 745-752.
ISSN 0966-0844
Abstract
The antimicrobial drug candidate 1,3-
dibenyl-4,5-diphenyl-imidazol-2-ylidene silver(I) acetate
(SBC3) was evaluated for its ability to function
in vivo using larvae of Galleria mellonella. A SBC3
concentration of 25 lg/ml inhibited the growth of
Staphylococcus aureus by 71.2 % and Candida albicans
by 86.2 % in vitro. Larvae inoculated with 20 ll
of SBC3 solution showed no ill effects up to a
concentration of 250 lg/ml but administration of
500 lg/ml resulted in a 40 % reduction in larval
survival and administration of a dose of 1,000 lg/ml
resulted in total larval death at 24 h. Larvae inoculated
with S. aureus or C. albicans and subsequently
administered SBC3 showed increased survival. Administration
of SBC3 to larvae did not boost the insect
immune response as indicated by lack of an increase in
the density of circulating haemocytes (immune cells).
The abundance of a number of proteins involved in the
insect immune response was reduced in larvae that
received 20 ll SBC3 solution of 100 lg/ml. This is the
first demonstration of the in vivo activity of SBC3
against S. aureus and C. albicans and demonstrates that SBC3 does not stimulate a non-specific immune
response in larvae.
| Item Type: |
Article
|
| Additional Information: |
The definitive published version of this article is available at DOI: 10.1007/s10534-014-9766-z |
| Keywords: |
Antimicrobial drug; Candida albicans; Carbene-silver complex; Galleria mellonella; In vivo
toxicity; Staphylococcus aureus; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
6896 |
| Identification Number: |
https://doi.org/10.1007/s10534-014-9766-z |
| Depositing User: |
Dr. Kevin Kavanagh
|
| Date Deposited: |
20 Jan 2016 13:19 |
| Journal or Publication Title: |
Biometals |
| Publisher: |
Springer Verlag |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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