Gannon, Jay and Ohlendieck, Kay
(2012)
Subproteomic analysis of basic proteins in aged skeletal muscle
following offgel pre-fractionation.
Molecular Medicine Reports, 5 (4).
pp. 993-1000.
ISSN 1791-2997
Abstract
The progressive loss of skeletal muscle mass is a
serious pathophysiological problem in the elderly, which
warrants detailed biochemical studies into the underlying
mechanism of age-related fiber degeneration. Over the last
few years, mass spectrometry (MS)-based proteomics has
identified a considerable number of new biomarkers of muscle
aging in humans and animal models of sarcopenia. However,
interpretation of the proteomic findings is often complicated
by technical and biological limitations. Although gel
electrophoresis-based approaches represent a highly sensitive
analytical way for the large-scale and high-throughput survey
of global changes in skeletal muscle proteins during aging,
often the presence of components with an isoelectric point in
the basic range is underestimated. We, therefore, carried out
a comparative subproteomic study of young versus aged rat
muscle focusing on potential changes in muscle proteins with
an alkaline isoelectric point, using a combination of offgel
electrophoresis and two-dimensional (2D) slab gel electrophoresis.
Offgel electrophoresis was successfully applied as
a prefractionation step to enrich basic protein species from
crude tissue extracts representing young adult versus senescent
muscle specimens. Proteomics has demonstrated alterations
in a small cohort of basic proteins during muscle aging. The
mass spectrometric identification of altered proteins and
immunoblotting revealed a decrease in the glycolytic enzyme
glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and a concomitant increase in mitochondrial creatine kinase (CK)
and ubiquinol cytochrome‑c reductase. This agrees with the
idea of a glycolytic-to-oxidative shift during muscle aging,
which is indicative of an overall fast-to-slow transition process
in senescent rat muscle. Thus, alterations in the abundance
of metabolic enzymes appear to play a central role in the
molecular pathogenesis of age‑dependent muscle wasting.
| Item Type: |
Article
|
| Additional Information: |
GANNON J, OHLENDIECK K. Subproteomic analysis of basic proteins in aged skeletal muscle following offgel pre-fractionation. Molecular Medicine Reports. 2012;5(4):993-1000. doi:10.3892/mmr.2012.759. |
| Keywords: |
aging; muscle aging; muscle proteomics; offgel electrophoresis; sarcopenia; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
6912 |
| Identification Number: |
https://doi.org/10.3892/mmr.2012.759 |
| Depositing User: |
Prof. Kay Ohlendieck
|
| Date Deposited: |
21 Jan 2016 14:37 |
| Journal or Publication Title: |
Molecular Medicine Reports |
| Publisher: |
Spandidos Publications |
| Refereed: |
Yes |
| Funders: |
Science Foundation Ireland (SFI), Higher Education Authority (HEA) |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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