Colabardini, Ana Christina and Humanes, Ana Carolina and Fagundes Gouvea, Paula and Savoldi, Marcela and Goldman, Maria Helena S. and von Zeska Kress, Marcia Regina and Bayram, Ozgur and Gomes, Marcelo Damario and Braus, Gerhard H. and Goldman, Gustavo H. (2012) Molecular characterization of the Aspergillus nidulans fbxA encoding an F-box protein involved in xylanase induction. Fungal Genetics and Biology, 49 (2). pp. 130-140. ISSN 1087-1845

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Abstract

The filamentous fungus Aspergillus nidulans has been used as a fungal model system to study the regulation of xylanase production. These genes are activated at transcriptional level by the master regulator the transcriptional factor XlnR and repressed by carbon catabolite repression (CCR) mediated by the widedomain repressor CreA. Here, we screened a collection of 42 A. nidulans F-box deletion mutants grown either in xylose or xylan as the single carbon source in the presence of the glucose analog 2-deoxy-D-glucose, aiming to identify mutants that have deregulated xylanase induction. We were able to recognize a null mutant in a gene (fbxA) that has decreased xylanase activity and reduced xlnA and xlnD mRNA accumulation. The DfbxA mutant interacts genetically with creAd-30, creB15, and creC27 mutants. FbxA is a novel protein containing a functional F-box domain that binds to Skp1 from the SCF-type ligase. Blastp analysis suggested that FbxA is a protein exclusive from fungi, without any apparent homologs in higher eukaryotes. Our work emphasizes the importance of the ubiquitination in the A. nidulans xylanase induction and CCR. The identification of FbxA provides another layer of complexity to xylanase induction and CCR phenomena in filamentous fungi.

Item Type:	Article
Additional Information:	The definitive published version of this article is available at DOI: 10.1016/j.fgb.2011.11.004
Keywords:	Aspergillus nidulans; Xylanase; Catabolite repression; CreA XlnR; F-box proteins; Xylanase;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	6947
Identification Number:	https://doi.org/10.1016/j.fgb.2011.11.004
Depositing User:	Ozgur Bayram
Date Deposited:	03 Feb 2016 10:40
Journal or Publication Title:	Fungal Genetics and Biology
Publisher:	Elsevier Inc.
Refereed:	Yes
URI:	http://www.sciencedirect.com/
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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