Proteomic Profiling of Cereal Aphid Saliva Reveals Both Ubiquitous and Adaptive Secreted Proteins


Rao, Sohail A.K. and Carolan, James C. and Wilkinson, Thomas L. (2013) Proteomic Profiling of Cereal Aphid Saliva Reveals Both Ubiquitous and Adaptive Secreted Proteins. PLoS ONE, 8 (2). e57413. ISSN 1932-6203

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Abstract

The secreted salivary proteins from two cereal aphid species, Sitobion avenae and Metopolophium dirhodum, were collected from artificial diets and analysed by tandem mass spectrometry. Protein identification was performed by searching MS data against the official protein set from the current pea aphid (Acyrthosiphon pisum) genome assembly and revealed 12 and 7 proteins in the saliva of S. avenae and M. dirhodum, respectively. When combined with a comparable dataset from A. pisum, only three individual proteins were common to all the aphid species; two paralogues of the GMC oxidoreductase family (glucose dehydrogenase; GLD) and ACYPI009881, an aphid specific protein previously identified as a putative component of the salivary sheath. Antibodies were designed from translated protein sequences obtained from partial cDNA sequences for ACYPI009881 and both saliva associated GLDs. The antibodies detected all parent proteins in secreted saliva from the three aphid species, but could only detect ACYPI009881, and not saliva associated GLDs, in protein extractions from the salivary glands. This result was confirmed by immunohistochemistry using whole and sectioned salivary glands, and in addition, localised ACYPI009881 to specific cell types within the principal salivary gland. The implications of these findings for the origin of salivary components and the putative role of the proteins identified are discussed in the context of our limited understanding of the functional relationship between aphid saliva and the plants they feed on. The mass spectrometry data have been deposited to the ProteomeXchange and can be accessed under the identifier PXD000113.

Item Type: Article
Additional Information: © 2013 Rao et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Financial support was provided through Department of Agriculture, Food and the Marine Research Stimulus Grant 07/553, and in part by Science Foundation Ireland Principal Investigator Grant 03/IN3/B381 and UCD Seed Funding SF268. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Keywords: Proteomic Profiling; Cereal Aphid Saliva; Ubiquitous Secreted Proteins; Adaptive Secreted Proteins; Sitobion avenae; Metopolophium dirhodum;
Academic Unit: Faculty of Science and Engineering > Biology
Item ID: 7172
Identification Number: 10.1371/journal.pone.0057413
Depositing User: James Carolan
Date Deposited: 07 Jul 2016 11:42
Journal or Publication Title: PLoS ONE
Publisher: Public Library of Science
Refereed: Yes
Funders: Department of Agriculture, Food and the Marine, Science Foundation Ireland (SFI)
URI:

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