More Than a Repair Enzyme: Aspergillus nidulans Photolyase-like CryA Is a Regulator of Sexual Development


Bayram, Ozgur and Biesemann, Christoph and Krappmann, Sven and Galland, Paul and Braus, Gerhard H. (2008) More Than a Repair Enzyme: Aspergillus nidulans Photolyase-like CryA Is a Regulator of Sexual Development. Molecular Biology of the Cell, 19 (8). pp. 3254-3262. ISSN 1059-1524

[img]
Preview
Download (1MB) | Preview
Official URL: http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E08...


Share your research

Twitter Facebook LinkedIn GooglePlus Email more...



Add this article to your Mendeley library


Abstract

Cryptochromes are blue-light receptors that have presumably evolved from the DNA photolyase protein family, and the genomes of many organisms contain genes for both types of molecules. Both protein structures resemble each other, which suggests that light control and light protection share a common ancient origin. In the genome of the filamentous fungus Aspergillus nidulans, however, only one cryptochrome/photolyase-encoding gene, termed cryA, was identified. Deletion of the cryA gene triggers sexual differentiation under inappropriate culture conditions and results in up-regulation of transcripts encoding regulators of fruiting body formation. CryA is a protein whose N- and C-terminal synthetic green fluorescent protein fusions localize to the nucleus. CryA represses sexual development under UVA350-370 nm light both on plates and in submerged culture. Strikingly, CryA exhibits photorepair activity as demonstrated by heterologous complementation of a DNA repair-deficient Escherichia coli strain as well as overexpression in an A. nidulans uvsBΔ genetic background. This is in contrast to the single deletion cryAΔ strain, which does not show increased sensitivity toward UV-induced damage. In A. nidulans, cryA encodes a novel type of cryptochrome/photolyase that exhibits a regulatory function during light-dependent development and DNA repair activity. This represents a paradigm for the evolutionary transition between photolyases and cryptochromes.

Item Type: Article
Additional Information: Creative Commons Attribution Non-Commercial Share Alike 3.0 License. Cite as Bayram O, Biesemann C, Krappmann S, Galland P, Braus GH (2008) 'More than a repair enzyme: Aspergillus nidulans photolyase-like CryA is a regulator of sexual development'. Molecular Biology of the Cell, 19 :3254-3262
Keywords: Repair Enzyme; Aspergillus nidulans; Photolyase-like CryA; Regulator; Sexual Development;
Academic Unit: Faculty of Science and Engineering > Biology
Item ID: 7183
Identification Number: 10.1091/mbc.E08-01-0061
Depositing User: Ozgur Bayram
Date Deposited: 13 Jul 2016 12:01
Journal or Publication Title: Molecular Biology of the Cell
Publisher: American Society for Cell Biology
Refereed: Yes
URI:

Repository Staff Only(login required)

View Item Item control page

Document Downloads

More statistics for this item...