Proteomic Profiling of Secreted Proteins from CHO Cells Using Surface-Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry


Kumar, Niraj and Maurya, Priyanka and Gammell, Patrick and Dowling, Paul and Clynes, Martin and Meleady, Paula (2008) Proteomic Profiling of Secreted Proteins from CHO Cells Using Surface-Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry. Biotechnology Progress, 24 (1). pp. 273-278. ISSN 8756-7938

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Abstract

Chinese hamster ovary (CHO) cells are the most commonly used host cell line for the production of recombinant biopharmaceuticals. These biopharmaceuticals are typically secreted from CHO cells and purified from harvested cell culture media. The purpose of this study was to investigate changes in the secreted proteome of CHO cells over the various stages of the growth cycle using Surface Enhanced Laser desorption ionization time-of-flight mass spectrometry (SELDITOF MS). Conditioned media samples were collected each day over a 6 day growth period from CHO-K1 cells grown in low serum (0.5% FBS) conditions in monolayer culture. Samples were profiled on a number of ProteinChip arrays with different chromatographic surfaces. From this study, 24 proteins were found to be differentially regulated at different phases of the growth cycle in CHO-K1 cells, when profiled on two chromatographic surfaces, Q10 (anionic) and IMAC30 (metal affinity) ProteinChip arrays.

Item Type: Article
Keywords: Proteomic Profiling; Secreted Proteins; CHO Cells; Surface-Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry; SELDITOF MS;
Academic Unit: Faculty of Science and Engineering > Biology
Item ID: 7353
Identification Number: 10.1021/bp070244o
Depositing User: Paul Dowling
Date Deposited: 17 Aug 2016 15:48
Journal or Publication Title: Biotechnology Progress
Publisher: American Chemical Society and American Institute of Chemical Engineers
Refereed: Yes
Funders: Science Foundation Ireland (SFI)
URI:

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