MURAL - Maynooth University Research Archive Library



    Does Calcium Turn a Zwitterionic Lipid Cationic?


    McManus, Jennifer and Rädler, Joachim O. and Dawson, Kenneth A. (2003) Does Calcium Turn a Zwitterionic Lipid Cationic? Journal of Physical Chemistry B, 107 (36). pp. 9869-9875. ISSN 1520-6106

    [img]
    Preview
    Download (144kB) | Preview


    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...



    Add this article to your Mendeley library


    Abstract

    The structure of a complex formed from DNA, calcium, and DPPC has been studied by small-angle X-ray scattering (SAXS). Previous DSC and temperature scanning ultrasound have shown that in excess lipid two DPPC phases exist, one corresponding to DNA bound lipid and the other uncomplexed lipid, not directly involved in complex formation.1,2 We have investigated the effect of calcium on both phases within the complex structure. Contrary to the observations in the ternary DPPC/calcium/water system, the binding of calcium to the DPPC membrane in this quaternary system containing DNA appears to be significantly enhanced. We have estimated the stoichiometry of calcium binding within the complex, and we concluded that the zwitterionic lipid is directly involved in binding to DNA.

    Item Type: Article
    Keywords: Calcium; Zwitterionic Lipid; Cationic; DNA; calcium; DPPC;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 7899
    Identification Number: https://doi.org/10.1021/jp034463d
    Depositing User: Jennifer McManus
    Date Deposited: 10 Feb 2017 15:44
    Journal or Publication Title: Journal of Physical Chemistry B
    Publisher: American Chemical Society
    Refereed: Yes
    URI:
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

    Repository Staff Only(login required)

    View Item Item control page

    Downloads

    Downloads per month over past year

    Origin of downloads